genetic analysis of three structural proteins in iranian infectious bronchitis virus isolate
Authors
abstract
infectious bronchitis virus (ibv) is a contagious pathogen in fowl that results in economic loss in the poultry industry. in this study, the amino acids sequences of three structural proteins m, n, and s1 for five iranian ibv isolated during 1998-2011 have been analyzed. conserved and variable regions, hydrophobic characteristics and identity matrix were determined after alignment by bioedit ver 7.0.4.1. the phylogenetic tree was obtained by using the neighbor-joining method within mega4. similarity for m and s1 protein was lowest for ir-3654-vm and tw2575 / 98 isolate (0.862) and ir1061-ph and georgia 1998 isolate / strain (0.41), respectively. for the n protein, highest similarity was found between ur1 / 09 and arkansas dpi (0.948). four conserved regions for m and n proteins were recognized. in the s1 protein three hypervariable regions were detected in 52-90, 124-150 and 265-315 residues. in a phylogeny analysis all proteins were distributed in three clusters. iranian ibv belonged to mass cluster in phylogeny tree of m and n proteins. but s1 protein showed a close relationship with the california serotype and was distantly related to mass serotype. the results showed that the iranian ibv isolate was probably diverted from mass strain that might be brought to iran as a vaccine strain.
similar resources
Genetic Analysis of Three Structural Proteins in Iranian Infectious Bronchitis Virus Isolate
Infectious bronchitis virus (IBV) is a contagious pathogen in fowl that results in economic loss in the poultry industry. In this study, the amino acids sequences of three structural proteins M, N, and S1 for five Iranian IBV isolated during 1998-2011 have been analyzed. Conserved and variable regions, hydrophobic characteristics and identity matrix were determined after alignment by Bioedit ve...
full textSequences and Phylogenetic Analysis of Envelope (E) Gene of Iranian Infectious Bronchitis Virus Isolates in Iran
Background and Aims: Infectious bronchitis virus (IBV) is the etiological agent of infectious bronchitis (IB), which is an acute and highly contagious disease of the respiratory and sometimes the urogenital tracts of chickens. In Iran, the disease has been identified in chicken flocks with serological and virus isolation methods. Gene 3c (E) encode envelop (small membrane) protein. For envelope...
full textSequence analysis of the VP1 gene in three very virulent Iranian infectious bursal disease virus strains
Infectious bursal disease (IBD) is a highly contagious disease of chickens caused by the infectious bursal disease virus (IBDV). This study was conducted to characterize three IBDV strains from Iran. A reverse transcriptase-polymerase chain reaction (RT-PCR) procedure was used to amplify a 715-bp fragment of the VP1 gene from IBDV strains. Amplified VP1 fragments of the three Iranian IBDV strai...
full textEmergence of variant avian infectious bronchitis virus in India
Background: Infectious bronchitis virus (IBV) is the etiological agent of an acute and highly contagious disease. Infectious bronchitis (IB) affects chicken of all ages and poses major economic loses to the poultry industry worldwide. The continuous evolution of the spike protein (S1) of IBV is responsible for the prevalence of many serotypes/genotypes around the world. Multipl...
full textVegad. Nucleocapsid Gene Sequence Analysis and Characterization of an Indian Isolate of Avian Infectious Bronchitis Virus
full text
Nucleocapsid Gene Sequence Analysis and Characterization of an Indian Isolate of Avian Infectious bronchitis virus
Avian Infectious bronchitis virus belongs to the family Coronaviridae. It is an enveloped virus with large positive stranded RNA genome. In the present study RNA was isolated from viral suspension and transcribed into cDNA. Poultry postmortem cases showing lesions of visceral gout were collected and infectious bronchitis virus were isolated. About 1.2 kb Nucleocapsid gene of virus was amplified...
full textMy Resources
Save resource for easier access later
Journal title:
iranian journal of applied animal sciencePublisher: islamic azad university - rasht branch
ISSN 2251-628X
volume 3
issue 3 2013
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023